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Store the reconstituted peptide in a refrigerator at 28C unless protocol requires otherwise

CT26 cells were purchased from the Chinese Academy of Sciences/Stem Cell Bank (Shanghai, China)

So instead of a serum, I may do something a little bit different to that

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Results Cryo-EM structure determination of OCTN2 To determine the structure of OCTN2, we overexpressed full-length human OCTN2 in HEK293F cells and isolated the transporter using a GFP-nanobody purification strategy 28