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Measurements of energy expenditure and locomotor activity HFD-induced obese C57BL/6J male mice were placed in individual small acryl calorimeter chambers with free access to HFD and water, and respiratory gas (O 2 and CO 2 ) were measured every 5 min for each individual mouse by indirect calorimetry system connected to mass spectrometer (Arco2000, ArcoSystem, Chiba, Japan)

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GIP is another neuroendocrine hormone that helps regulate blood sugar levels, insulin release, and insulin sensitivity

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cDNA counts at individual iCLIP cross-link sites were normalized to the total number of reads in each experimental library and scaled to counts per million (CPM)