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Thus it is vital to follow a specific process within 30 minutes of collection: Collect blood plasma (using EDTA or heparin to prevent coagulation) and then centrifuge the sample to remove the blood cells from the plasma

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Sated rats received an ICV injection of OXM, GLP-1 (3 nmol), or saline at the onset of the dark phase

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Dinis-Oliveira, R.J