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aureus were stained with 100300 nM LysoTracker Green DND-26 (ThermoFisher) at 37 C for 30 min, then washed and maintained in 200 l complete MEM containing 1 g ml 1 Hoechst 33342, 50 g ml 1 Gen and 2 M aTc

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Dose Rate: Lambs: 0.5 mL at marking or weaning Sheep: 1.0 1.5 mL pre-lambing Calves: 2 3 mL from 2 months of age Cows: 4 6 mL pre-calving The frequency of dosing should be related to the severity of the deficiencies