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Briefly, 2 10 7 cells were lysed in 600 l of PLB buffer (100 mM KCl, 5 mM MgCl 2 , 10 mM HEPES pH 7.0 and 0.5% NP-40) with RNaseOUT recombinant ribonuclease inhibitor (Invitrogen, 10777019) and protease inhibitor (APExBIO, k1007) and 12 l of lysate was saved as input
doi:10.1046/j.1365-2125.2001.01305.x
Cng dng: Cch dng: Glutathione 600 Dr.Lacir c ngun nguyn liu nhp khu t Nht Bn , hin ang c rt nhiu ch em s dng v tnh an ton, hiu qu v gi thnh hp l
A 10-m size nebulizer was used for the exposures