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Protein samples were denatured and subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by transfer to a PVDF membrane (Millipore) using a constant current of 300 mA

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AST downregulates the expression of lipogenesis genes (such as Glycerol-3-phosphate dehydrogenase) and reduces triglyceride accumulation in 3T3-L1 adipocytes [92]