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The metabolites were extracted with 500 l of ice-cold MeOH/H 2 O (80:20, vol/vol) containing 0.01 M lamivudine and 1 M each of D 2 -glucose, D 4 -succinate, D 5 -glycine and 15 N-glutamate (Sigma-Aldrich)

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Diabetes represents a major public health challenge in Italy