After centrifugation, 300 L PBS was added to resuspend cells, followed by the addition of 700 L pre-cooled anhydrous ethanol drop by drop, and incubated at 4 for 1824 h
Polyak 2013
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It cannot cut proteins if proline is in the third position [5]
Patejunas G, Lee B, Dennis JA, Healy PJ, Reeds PJ, Yu H, Frazer M, Mull B, Warman AW, Beaudet AL, OBrien WE (1998) Evaluation of gene therapy for citrullinaemia using murine and bovine models