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For the screen, 300 10 6 MCF-7 iCas9 cells were infected at a multiplicity of infection (MOI) of 0.3, aiming for 1000 cells per individual sgRNA, with a previously published and validated genome-wide human lentiviral CRISPR library 17

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doi: 10.3390/cimb45080420 15 ZuoLPratherERStetskivMGarrisonDEMeadeJRPeaceTIet al

However, as earlier suggested neurotrophic agents possess characteristics that make them excellent candidates

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