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May 15 2013;309(19):2005-15

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This leads to the subsequent triggering of an inflammatory response and apoptosis, which accelerate the progression of steatohepatitis

Proteins were then separated via SDS-PAGE and transferred to PVDF membranes, which were blocked with 5% skim milk for 1 h at 25 C before incubation with primary antibodies for 12 h at 4 C