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Avoid Freezing Reconstituted Peptides: Freezing can cause the peptide to degrade, especially if it undergoes repeated freeze-thaw cycles

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Mitochondrial D 9 -TML uptake assays Confluent 15 cm dishes of differentiated SVF derived from Ing WAT were washed twice with ice-cold PBS then collected in ice-cold KPBS (136 mM KCl, 10 mM KH 2 PO 4 , pH 7.25)