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After deparaffinization, sections were heated in an autoclave at 121 C for 5 min in 10 mM sodium citrate buffer (pH 6.0) or reacted with protease solution (Nichirei Biosciences, Tokyo, Japan) for epitope retrieval, after which endogenous peroxidase was inactivated by 0.3% hydrogen peroxide in methanol

(2007) 292:L51928

First, the components cannot be separated after reconstitution, so protocols needing an independently chosen ratio require separate vials

Solution: Temporary lumps need no treatment

Temperature accelerates this process