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Teneligliptin demonstrates 5 times greater activity compared to sitagliptin [8]
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Cells were then lysed in 1X TBS (50 mM Tris-HCl, 150 mM NaCl, pH 7.4) supplemented with 1 mM EDTA, 1% Triton X-100, phosphatase inhibitor cocktail (P5726, Sigma Aldrich), and protease inhibitor cocktail (11873580001, Roche Diagnostics)
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This controlled copper delivery enables safe intracellular uptake without the toxic effects of free copper