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Chromatographic runs were performed with methanol/water (80%/20%) and acetate-buffered methanol (pH = 4.6) as mobile phases and with fluorescent measurement at 335 nm of excitation and 518 nm of emission

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Other hormones, such as thyroid hormone and pregnenolone, work continuously to regulate metabolism and overall balance

10.1056/nejm199004123221502 50 KawamuraE.EnomotoM.KotaniK.HagiharaA.FujiiH.KobayashiS.et al (2012)

Stopping them in between can result in increased blood sugar and weight gain once again