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Next increasing volumes (2, 5, 10, 15, 20, 25 L) of the solution of 0.250 mol/L TCEP were added to the samples to obtain the required molar excess of TCEP in relation to N-Hcy-Lys (50:1, 125:1, 250:1, 375:1, 500:1, 625:1)

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By following these guidelines, healthcare providers and patients can ensure effective treatment