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The selective inhibition of iNOS reduces the tissue level of catabolic factors, so NO plays an inflammatory role in OA (Scher et al., 2007)

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The cell pellets were then resuspended and transferred to a Matrigel-coated surface in induction medium (IM) (DMEM/F12 (Gibco, 11320033) supplemented with N2 supplement (Gibco, 17502048), L-glutamine (Gibco, 25030081), MEM NEAA (Gibco, 11140050), and 2 g/mL doxycycline (Sigma, D9891)) supplemented with 10 M Y-27632 ROCK inhibitor (Abcam, ab120129)

The risk calculus changes accordingly