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The total duration of anesthesia was approximately 10 min, and injections were conducted at room temperature (22C)

Without IGFBP interference, LR3 remains active in circulation for 20-30 hours : nearly 100 times longer than natural IGF-1

After a 15-min incubation in the dark, the percentage of Annexin V pos and PI pos cells was analysed using the FL-1 and FL-3 channels on an Accuri C6 Flow Cytometer (BD Biosciences)

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The oral small-molecule inhibitor INCB001158 (CB-1158), targeting this axis, has entered first-inhuman phase I studies and has been explored in combination with PD-1 inhibitors, suggesting that relieving substrate depletion-driven immunosuppression is clinically feasible from a safety and pharmacodynamic perspective, although its therapeutic efficacy remains to be fully established (116)