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The elevated plasma adenosine is believed to reflect both (a) increased plasma accumulation of ATP from RBCs themselves or tissues, and (b) activity of the ectonucleotidases CD39 and CD73, which hydrolyze ATP, leading ultimately to the formation of adenosine

They were removed to 25C and were shaken overnight

The enzyme BHMT, which contains zinc, makes this process happen using the methyl groups from TMG, which comes from food or from the breakdown of choline

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