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Even if the expired injection looks clear and unchanged, molecular degradation happens invisibly at the molecular level

I also used a night splint for consistent overnight stretching

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Treatment of mice with 2APB 2APB (Sigma, D9754) was made fresh for each experiment (dissolved in DMSO at 100 mg/ml as the stock)

The research team found that nicotinamide (Nam) in CMAs worked best to increase NAD+ products

Slide backs were tapped on padding and dried with paper towels and then placed section side up on a benchtop slide warmer, setting 4 for 1 h or until sections were opaque and all droplets had dissolved