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Hippocampal neurons were maintained in Neurobasal A media from Invitrogen (Carlsbad, CA) supplemented with B27 from Invitrogen 0.5 raM L-glutamine, and 5mM cytosine-D-arabinofuranoside from Sigma added at 2 days in vitro

The taper protocol applies specifically to peptides that stimulate or amplify endogenous hormone release

More specifically, IGF-1 LR3 is an 83 amino acid analog of IGF-1 comprising the complete human IGF-1 sequence with the substitution of an Arg (R) for the Glu(E) at position three, and a 13 amino acid extension peptide at the N terminus from methionyl porcine Growth Hormone [1]

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Mar 1982;31(3):249-254