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Cytokine levels were measured using enzyme-linked immunosorbent (ELISA) assay kits: Human 1L-10 ELISA Ready-SET-Go from Affymetrix (Cat# 88-7106) and Human TNF- Uncoated ELISA (Cat # 88-7346), Human IFN- Uncoated ELISA (Cat # 88-7316), and Human IL-6 Uncoated ELISA (Cat # 88-7066) from Invitrogen to quantity detection of cytokines

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The audit trailwhich captures the "metadata" of the analysis (e.g., how many times a sample was injected, whether integration parameters were changed)is part of that record

In line with these findings, we discovered that SFN could inhibit the expression of IL-6 and IL-1, both of which are regulated by NRF2 [47, 48]

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it is a single-use diluent meant to be used immediately after opening and not stored for reuse