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Isolated DTCs were transferred into 200 l PBS with 0.1% BSA and further collected into ~4 l PBS with 0.1% BSA

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Gauge (Needle Thickness) The gauge refers to the thickness of the needle

Saline was provided to control mice in the same volume

Improving the sensitivity During the analysis of biological samples after non-invasive semaglutide administration, the LLOQ was optimized to 0.4 ng/mL by enhancing the signal and reducing background noise (Figure 5)

Whole grains: The carnivore diet prohibits all whole grains, including brown rice, whole-wheat bread and pasta, and oatmeal