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A phase II randomized placebo-controlled trial

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At the end point, cells were collected by trypsinization (the culture medium and PBS washes were collected and re-added to each sample) and washed once with assay buffer (10 mM HEPES, 140 mM NaCl and 3.3 mM CaCl 2 , pH 7.4)

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17 Any distortion of these properties is termed endothelial dysfunction, a phenomenon that can be easily demonstrated as an impaired vasorelaxation in response to endothelium-dependent vasodilators such as acetylcholine

Chung C et al (2023): New Practice Guidelines for Neuromuscular Blockade