In all cases, the ligand: receptor mixtures were incubated for 30 min at 15 C and the HTRF signal recorded using a Cary Eclipse spectrofluorimeter (Varian) with an excitation wavelength set at 337 nm and an emission wavelength at 665 and 620 nm
From a chemical compatibility perspective, adding bacteriostatic water to a third-party peptide can produce unpredictable results if the peptide material has an unsuitable pH, contains residues from manufacture, has degraded during storage, or has poor solubility characteristics
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