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Recently, TMEM164 (transmembrane protein 164) was identified as a mediator of ferroptosis, with multifunctional roles as an acyltransferase involved in the synthesis of C20:4 ether phospholipids in renal carcinoma 786-O cells [97] or in driving the formation of phagophores, leading to excessive autophagosome formation in HT1080 and pancreatic cancer cell line PANC1 [98]

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Alabdali A, Bacha AB, Alonazi M, Al-Ayadhi L, Alanazi AS, El-Ansary A (2025) Comparative Evaluation of Certain Biomarkers Emphasizing Abnormal GABA Inhibitory Effect and Glutamate Excitotoxicity in Autism Spectrum Disorders

[33] report that rats fasted for 48 hours lose approximately 44% of the intracellular GSH in their hepatocytes

The latter treatment did solubilize the recombinant proteins to some extent (Fig