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The immunofluorescence results also revealed that the number of lysosomes did not increase (Supplementary Fig

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doi:10.1073/pnas.1733391100

Lane 1: Molecular weight marker Lane 2: Cell pellet after sonication Lane 3: Supernatant after sonication Lane 4: Flow through after binding Lane 5: Wash Lane 6: Elution 1 Lane 7: Elution 2 Lane 8: Elution 3 Lane 9: Elution 4 Lane 10: Elution 5 GST-E2-25K was purified with Glutathione Agarose Resin using a gravity column

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