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Elimination is achieved by means of a mobile phase, typically combination of water and organic solvents (such as acetonitrile or methanol) are sprayed onto the column in the form of gradient, such as 95:5 aqueous: organic, for example and gradually increasing the organic phase until the elution buffer is 5:95 organic: aqueous)

Aging Cell 13 , 273282, doi:10.1111/acel.12170 (2014)

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10.1016/s0140-6736(03)13555-6 247 UmedaT.KimuraT.YoshidaK.TakaoK.FujitaY.MatsuyamaS.et al (2017)

[PMID:25824437] Filler RM, Forte V, Chait P