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While our qPCR analysis relied on a single reference gene (GAPDH) that showed stable expression, future studies could benefit from incorporating multiple reference genes as recommended by the MIQE guidelines [51]

Lamers D, Famulla S, Wronkowitz N, Hartwig S, Lehr S, Ouwens DM, et al

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Start with simple bodyweight exercises at home, like squats, push-ups, and jumping jacks, and gradually incorporate short bursts of high-intensity activities like sprinting in place or fast cycling

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