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Briefly, sitting drop crystallisations were set up in 96-well trays using a Cartesian robot with a reservoir solution of 1.5 M ammonium sulfate and 0.1 M Tris at pH 8.5, each crystallisation drop containing 100 nL each of protein and reservoir solutions

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Maintain your regular 7-day dosing interval based on your original schedule, and adjust the calendar day according to your destination time zone

1996 Oct;19:129-39

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