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Peptidoglycan (PGN) is the main component of the bacterial cell wall, especially for Gram positive bacteria [5], whilst muramidases (EC 3.2.1.17) of the class hydrolases, also known as lysozymes, catalyze the hydrolysis of the PGN at -1, 4 glycosidic linkages level, between N-acetylmuramic acid and N-acetyl-D-glucosamine residues [6, 7]
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Notably, the pK of the resulting metal-NA complexes as well as the pH of the solution (with most metals being chelated at neutral or basic pH values) were considered to significantly control the ability of NA to chelate metals (Relln-lvarez et al., 2008)