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For confocal fluorescence imaging of FSP1, sfGFP was fused to the C-terminus of FSP1 with a flexible linker of GGSGG using overlap PCR

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Biohacker Protocol (experimental) Dose: 0.1 0.2 mg (= 48 clicks) Duration: 8 12 weeks Frequency: 2 per week Cycle Interval: 2 4 weeks off before repeating Goal / Description: Lower-dose continuous design exploring sustained anabolic signaling