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20 After treating cultured sebocytes with IGF-1, a study performed polymerase chain reaction and enzyme-linked immunosorbent assay to evaluate changes in the expression of inflammatory markers such as interleukin (IL) 1, IL-6, IL-8, tumor necrosis factor , and nuclear factor kappa-light-chain-enhancer of activated B cells

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In addition, the decrease of KGDHC immunostaining was roughly related to the severity of degeneration