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Loading of RAW264.7 cells with oxidized low-density lipoprotein followed by LPS stimulation elevated GSR expression, and this correlated with a more rapid drop in cytosolic and mitochondrial NADPH in our assay

It has been shown that pharmacological inhibition or knockdown of a single mGSH transporter significantly sensitized neurons to oxidative and nitrosative stress (Wilkins et al., 2013)

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Mohan ME, Thomas JV, Mohan MC, Das SS, Prabhakaran P, Pulikkaparambil Sasidharan BC

Wipe the surface down and lay out all supplies before touching anything else

This method is ideal for systemic circulation or when the injury is not localized