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The clock starts with that first breach of the sterile seal

The GATEWAY transformation system (Thermo Fisher Scientific) was used to convert the inserted fragment into an n-terminal 3xFLAG, His, HA, GST, or Myc labeled destination vector (for expression in mammalian cells)

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Pre-clinical research conducted primarily in animal models has studied its potential roles in supporting the GI environment, gut barrier function, and inflammatory responses in the GI tract

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doi: 10.1016/j.freeradbiomed.2023.09.005 186 FrickAKhareVPaulGLangMFerkFKnasmllerSet al