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Reactions were prepared by incubating 16 nmol l 1 DPP-4 (D3446, Aldrich) at 37 C, with a final test article concentration of 0.1 mmol l 1 in sterile Tris HCl buffer (50 mmol l 1 , pH 7.4)

doi: 10.1136/gutjnl-2018-316723 18 CaniP

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The lower lip, in particular, receives years of unprotected sunlight in most people because lip sunscreen is rarely applied with the same diligence as facial sunscreen

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