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Calculate the recovery by comparing the measured value with the expected amount of GLP-1 in the sample

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In addition, GLP-1 is also a neuropeptide produced by neurons in the lower brainstem 4,5,6 that suppress eating in rodents 7,8,9,10,11

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These pseudo-observations serve to anchor the doseresponse relationship at a physiologically plausible baseline (i.e., no treatment effect in the absence of exposure), thereby preventing unrealistic extrapolation near zero dose

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