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The PINK1-PARKIN mitochondrial ubiquitylation pathway drives a program of OPTN/NDP52 recruitment and TBK1 activation to promote mitophagy

Recent in vivo measurements of the brain and blood GSH may shed light on GSH changes earlier in the disease

In order to recover GST-tagged protein from the complicated mixture of proteins, the fusion protein is incubated with agarose beads coupled to glutathione (GSH-agarose, see picture below), what leads to efficient precipitation of GST-fusion protein

The controls we used were reduced forms of Trx-like enzymes and other proteins known to be modified by GSH