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Cell culture and treatment Immortalized human keratinocyte (HaCaT) cells (NE Fusenig, Heidelberg, Germany) were cultured in adipocyte medium (AM, high-glucose DMEM with 10% FBS and 1% P/S) at 37 C in a humidified incubator with 5% CO 2

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Glucagon-like peptide-1, commonly known as GLP-1, is a naturally occurring hormone produced in the gut and brain

The overall evidence of the impact of vitamin E on fibrosis remains limited, suggesting that its main role may be in addressing early-stage liver damage rather than reversing advanced fibrosis