For the RAW Blue NF-B assay, 10 5 RAW Blue cells (Invivogen, raw-sp) were plated per well in clear bottom 96-well plates
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Cells were separated from muscle tissue debris by differential centrifugation and filtration (nylon cell strainers) prior to a final centrifugation step at 1500 x g for 3 min, then suspended in DMEM containing 10% normal horse serum (HS), 1% antibiotic-antimycotic mixture, and 0.5% gentamicin (DMEM-10% HS), and plated on poly-L-lysine and fibronectin-coated plates
CAS Number: 89030-95-5
Excessive consumption of over-the-counter drugs containing paracetamol (eg, antipyretics, analgesics, etc.) can lead to hepatotoxicity and deplete the bodys glutathione system
Human APOC3 gene regulatory regions contain a set of proximal promotor with four elements ( 283/+ 24) and distal enhancer with six elements ( 890/ 500) [9]