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Flow cytometry was performed on a LSRFortessa X-20 (Becton Dickinson, Franklin Lakes, NJ) and data were analyzed using FlowJo (v7.1.6, TreeStar)

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Providers should screen for cobalt allergy beforehand and have epinephrine and emergency protocols available on-site

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Hydrogen peroxide solution (H 2 O 2 3%) was added to the slides to prevent endogenous peroxidase activity, followed by primary antibodies (anti-BAX 1:100) (ABclonal , USA) and (anti-GFAP 1:100) (Diagnostic BioSystems, USA) to be incubated with the slides overnight