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Finally, 50 L suspension buffer was used to re-suspend and obtain complete and unbroken nuclei

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They both consisting of: An extracellular N-terminus that binds the C-terminal part of the incretin analog A seven transmembrane helical domain (TMD) with the a-helices separated by three intracellular loops and three extracellular loops An intracellular C-terminus that is responsible for signaling

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