GE was analyzed through an isoperibol calorimeter system using benzoic acid as an internal standard (model 1261, Parr Instruments, Moline, Illinois, USA) at Pemberton Analytical Services (Shropshire, UK)
Notably, hydrogen bonds, which account for the high affinity, are formed between DEHP and proteins due to the four hydrogen bond receptors of the carboxyl groups, including ASN-51 of HSP90AA1, ARG-24 of IL6, LYS-54 of MAPK1, GLN-20 of BCL2, GLN-20 of EP300, ARG-207 of CASP3, GLN-68 and THR-45 of ERBB2, LYS-87 of BCL2L1, and ARG-112 of GRB2
Pinch a small skinfold to access subcutaneous tissue
PMID 16203922
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