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Second, larger peptides would give complex mixtures which would have to be separated and painstakingly examined to find suitable pieces for overlapping

Accordingly, the obtained formazan was measured photometrically at 550 nm with 690 nm as reference wavelength, by using a microplate reader (Synergy H1, BioTek, Bad Friedrichshall, Germany)

Hua X, Deng R, Li J, Chi W, Su Z, Lin J, Pflugfelder SC, Li DQ

Zeginiadou, T., Symeonidis, E

On the day of the experiment, the slides were removed from the freezer and SPZs were blocked with 1% BSA in PBS for 30 min at RT

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