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The 2017 Neelakantan paper in Biochemistry characterised a fluorescent assay for direct real-time monitoring of NNMT enzymatic activity, providing the analytical foundation for inhibitor screening including 5-Amino-1MQ (PMID 28121423)

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The oxidative pathway produces N-acetyl-p-benzoquinone imine (NAPQI), a highly reactive metabolite capable of binding cellular proteins and causing hepatocyte death

GCL, Glutamate-Cysteine Ligase

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