For most small peptides, solid-phase peptide synthesis (SPPS) remains a practical and flexible approach, offering access to non-natural modifications and greater process predictability
200 g Fmoc-Gly-Wang Resin (21.6 mmol) with a degree of substitution of 0.108 mmol/g was weighed and swelled for 30 min with DMF, followed by sequentially coupling according to the amino acid sequence of Semaglutide with 2-5 equivalents of amino acid feeds and condensing with DIC+A or B+A+C, until the resin was detected to be transparent with ninhydrin, wherein A was HOBt or HOAt
Target peak components were collected to obtain refined peptides with a purity of more than 98.5%
and the solvent is chosen from one or a mixture of two or more of DMF, CH 2 Cl 2 , NMP, or DMSO
Procd de prparation selon l'une quelconque des revendications 1 8, dans lequel le clivage dans l'tape 2 est ralis avec un ractif comprenant un ou un mlange de deux ou plus de TFA, PhSMe, PhOMe, EDT, H 2 O, TIS ou PhOH
GIP (Glucose-dependent Insulinotropic Polypeptide) and GLP-1 (Glucagon-Like Peptide-1) are both important hormones released after eating to help regulate your insulin levels, but they have some key differences
