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PVDF membranes were blocked with tris-buffered saline with Tween 20 (10 mM TrisHCl, pH 7.4, 150 mM sodium chloride, 0.05% Tween 20) containing 2% skim milk (Fujifilm Wako Pure Chemical Corp.) and then incubated with a 1:6,000 dilution with rabbit anti-domestic cat cauxin antibody followed by a 1:6,000 dilution with horseradish peroxidase-conjugated goat anti-rabbit immunoglobulin G (BioRad Laboratories, Inc.)

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10.1016/j.bbr.2009.06.035 [DOI] [PubMed] [Google Scholar] Akarte A