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Frozen tissues were thawn, carefully minced in lysis Buffer (10 mM Tris-Hcl, 146 mM NaCl, 21 mM MgCl 2 , 1 mM CaCl 2 , 0.1% CHAPs pH = 7.4) and grinded by using a tight douncer (Thermo Fisher Scientific, Waltham, MA, United States) on ice