Using ImageJ and constant parameters for each experiment, background was subtracted, local contrast was enhanced by using CLAHE 60 , and thresholding was performed
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However, the potential for delaying or preventing the progression of T2DM in patients treated with GIP and GLP-1 remains uncertain, as there is currently no definitive evidence from human studies demonstrating that enhanced incretin activity directly affects -cell mass or alters the progressive loss of insulin secretory capacity in humans
441 Analytical Methodology Both enzymatic and chemical methods are used to measure ammonia in body fluids
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WW, XD, ZH, and QP involved in drafting the manuscript