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Mouse sperm collection and incubation The non-capacitating medium used in this study was a modified ToyodaYokoyamaHosi (modified TYH) containing 119.3 mM NaCl, 4.7 mM KCl, 1.71 mM CaCl 2 .2H 2 O, 1.2 mM KH 2 PO 4 , 1.2 mM MgSO 4