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Ipamorelin and Growth Hormone Secretion Pathways In vitro studies examining pituitary somatotrophs suggest that Ipamorelins interaction with GHS-R may trigger a cascade of intracellular signaling events .(3) Proposed mechanisms include: Activation of phospholipase C (PLC) Increased production of second messengers inositol triphosphate (IP) and diacylglycerol (DAG) IP-mediated release of Ca from intracellular stores DAG-mediated activation of protein kinase C (PKC) The combined rise in intracellular calcium and PKC activation is thought to promote exocytosis of GH-containing vesicles from anterior pituitary cells